Questions: In the gel filtration experiment, why was Sephadex G-100 used? Explain why there are only two peaks, yet there are three proteins in the mixture, and account for the difference in peak size? In the anion-exchange experiment explain the elution of protein 2 (hint: think about the charge of the protein at the pH that was used)? Exercise 2 In order to start a new exercise you will need to select Quit, then Start Again from the Beginning. In this exercise you will use information ob …
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